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DSIP is administered at Age Management of West Michigan by sublingual spray that is administered under the tongue to optimize absorption and promote positive treatment response at lower dosages
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Wash buffer contained 10 mM Tris-HCl (pH 7.4), 10 mM NaCl, 3 mM MgCl 2 , 1% BAS, 0.1% Tween-20, 1 mM DTT (MilliporeSigma, 646563) and 1 U µl –1 RNase inhibitor (MilliporeSigma, 3335399001) in nuclease-free water
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